µÎÁ¡¹ÚÀ̻罿¹ú·¹ Prosopocoilus blanchardi (Parry,1873)

|
¢Ã ±¹ ¸í : µÎÁ¡¹ÚÀ̻罿¹ú·¹
¢Ã ÇÐ ¸í : Prosopocoilus blanchardi Parry,1873
¢Ã ÁöÁ¤¹øÈ£ : ¸êÁ¾À§±â ¾ß»ý»ý¹° ¥±±Þ
¢Ã °è Åë : ÀýÁöµ¿¹°¹® > °ïÃæ° > µüÁ¤¹ú·¹¸ñ > »ç½¿¹ú·¹°ú > µÎÁ¡¹ÚÀ̻罿¹ú·¹¼Ó
|
¡Ý Çü Å : ¸öÀº Ȳ°¥»ö ¶Ç´Â ¿¬ÇÑ °¥»öÀ̸ç, ¾Õ°¡½¿µîÆÇ °¡¿îµ¥ÀÇ °¡´Â ¼¼·Î ÁÙ°ú ¾ç¿·ÀÇ µÚÂÊ¿¡ ÀÖ´Â µÕ±Ù ¹«´Ì, µüÁö³¯°³ÀÇ °¡¿îµ¥¿Í °¡ÀåÀÚ¸®ÀÇ °¡´Â ÁÙ µîÀº Èæ»öÀÌ´Ù. ¼öÄÆÀº ¸ö±æÀ̰¡ Å«ÅÎÀ» Æ÷ÇÔÇÏ¿© 47-60 mm °¡·®ÀÌ´Ù. Å«ÅÎ(ÀÌ»¡)Àº ¸Å¿ì ±æ¸ç °¡´Ã°í, ¾à°£ ¾Æ·¡ÂÊÀ¸·Î ±Á¾úÀ¸¸ç, ¹Ù±ùÂÊÀº ³Ð°Ô µÕ±Û´Ù. Å«ÅÎÀÇ ¾ÈÂÊ ±âºÎ ±Ùó¿¡´Â ³Ð°í »ÏÁ·ÇÑ ÀÌ»¡ÀÌ ÀÖ°í, ³¡ºÎºÐ¿¡´Â 4°³ Á¤µµÀÇ ³¯Ä«·Î¿î ÀÌ»¡ÀÌ ÀÖ´Ù. ¾ÏÄÆÀº ¸ö±æÀ̰¡ 23-24 mm °¡·®ÀÌ´Ù.

¡Ý »ý Å : »ó·ÏȰ¿±¼ö¸²´ë¿¡¼ »ýȰÇϸç, Ȱ¿±¼öÀÇ ¼ö¾×À» »¡¾Æ¸Ô´Â´Ù. ¼ºÃæÀº ÁÖ·Î ¹ã¿¡ Ȱµ¿Çϸç, ³·¿¡´Â ³«¿±À̳ª Åä¾ç ¼Ó¿¡¼ ÈÞ½ÄÀ» ÃëÇÑ´Ù. ¾ÏÄÆÀº 6~8¿ù¿¡ »ê¶õÀ» Çϸç, ½âÀº ³ª¹«¿¡ 3~7mm Á¤µµÀÇ È¨À» ÆÄ¼ ¾ËÀ» ³º°í ³ª¹« ºÎ½º·¯±â·Î ±¸¸ÛÀ» ¸Þ¿î´Ù. ¾ËÀº ¿©¸§¿¡ ¾à 2ÁÖÀÏ Á¤µµ ÈÄ¿¡ ºÎÈÇÑ´Ù. À¯Ãæ°ú ¼ºÃæ »óÅ·Π¿ùµ¿À» ÇÑ´Ù. »ö±òÀÌ ¿¹»Ú°í ´õµëÀ̸¦ ¶°´Â ½À¼ºÀÌ ÀÖ¾î °ïÃæ ¼öÁý°¡µéÀÌ ¼±È£ÇÑ´Ù. ´Ù¸¥ »ç½¿¹ú·¹µé°ú ¸¶Âù°¡Áö·Î Çü±¤ºÒºûÀ» ÁÁ¾ÆÇÑ´Ù. Çѱ¹¿¡¼´Â Á¦ÁÖµµ¿¡ ¼½ÄÇϴµ¥, ÀÚµ¿Â÷³ª °¡·Îµî ºû¿¡ ²ø·Á ³¯¾Æ¿Ô´Ù°¡ Á×´Â °æ¿ì°¡ ¸¹´Ù.
¡Ý ºÐ Æ÷ : Çѱ¹(Á¦ÁÖµµ), ¸ù°ñ, ŸÀÌ¿Ï, ³×ÆÈ, Áß±¹ µîÁö¿¡ ºÐÆ÷ÇÑ´Ù.
¨ç äÁýÁ¤º¸
- ä¡¡Áý¡¡ÀÚ : ºû°íÀ»»ýÅ¿¬±¸¼Ò Á¤Çåõ
- äÁýÁö¿ª : Á¦ÁÖÆ¯º°ÀÚÄ¡µµ ¼±ÍÆ÷½Ã

|

|
µÎÁ¡¹ÚÀ̻罿¹ú·¹ ¼½ÄÁö ¹× Æ÷ȹÀå¼Ò ȯ°æ
|
äÁýÇÑ µÎÁ¡¹ÚÀ̻罿¹ú·¹ 2°³Ã¼
|
¨è äÁýÇöȲ ¹× »ùÇÃó¸®
µÎÁ¡¹ÚÀ̻罿¹ú·¹ äÁýÇöȲ
|
»ùÇÃó¸®
|
äÁýÀÏ
|
°³Ã¼ ¼ö
|
DNAseq
|
RNAseq
|
Ç¥º»
|
2017.07.20
|
2 EA
|
1 EA
|
1 EA
|
¤Ñ
|
¨é NCBI Taxonomy À¯ÀüÁ¤º¸ ÇöȲ
À¯ÀüÁ¤º¸ Á¾·ù
|
Genus Prosopocoilus À¯ÀüÀÚ¿ø
|
Prosopocoilus blanchardi À¯ÀüÀÚ¿ø
|
Nucleotide
|
82
|
22
|
Protein
|
94
|
21
|
Genome
|
2
|
-
|
Gene
|
74
|
-
|
¨ê Sequencing °á°ú
Type
|
Total Reads
|
Total Bases
|
Total Bases (Gb)
|
GC Rate
|
ºñ°í
|
DNA
|
199,733,134
|
30,159,703,234
|
30.1
|
34.5
|
|
RNA
|
52,742,576
|
7,615,641,533
|
7.6
|
40.6
|
|
- Illumina DNA Sequencing °á°ú
SOAP de novo
|
Pb_DNA hiseq
|
Input information
|
¡¡
|
|
¡¡
|
Region1
|
Region2
|
Total
|
raw data
|
Number Of Reads
|
|
|
101,597,184
|
101,597,184
|
203,194,368
|
Number Of Bases
|
|
|
15,341,174,784
|
15,341,174,784
|
30,682,349,568
|
error correction
|
Number Of Reads
|
|
|
101,232,007
|
99,239,971
|
200,471,978
|
Number Of Bases
|
|
|
15,079,060,037
|
14,311,680,780
|
29,390,740,817
|
pair reads
|
Number Of Reads
|
|
|
|
198,290,468
|
198,290,468
|
Number Of Bases
|
|
|
|
29,087,376,322
|
29,087,376,322
|
single reads
|
Number Of Reads
|
|
|
|
2,181,510
|
2,181,510
|
Number Of Bases
|
|
|
|
303,364,495
|
303,364,495
|
Assembly Results
|
Scaffold Metrics
|
> 100 bp
|
> 500 bp
|
> 1000 bp
|
> 2000 bp
|
Number Of Scaffold
|
|
501,865
|
129,793
|
85,553
|
54,578
|
Number Of Bases
|
|
464,763,262
|
397,202,039
|
366,052,470
|
322,082,424
|
Avg. Scaffold Size
|
¡¡
|
926
|
3,060
|
4,278
|
5,901
|
N50 Scaffold Size
|
¡¡
|
4,603
|
5,801
|
6,410
|
7,362
|
N80 Scaffold Size
|
¡¡
|
891
|
2,111
|
2,779
|
3,791
|
N90 Scaffold Size
|
¡¡
|
282
|
1,171
|
1,815
|
2,863
|
Largest Scaffold Size
|
¡¡
|
64,281
|
64,281
|
64,281
|
64,281
|
Contigs
|
> 0 bp
|
> 100 bp
|
> 500 bp
|
> 1000 bp
|
> 2000 bp
|
Number Of Contigs
|
11,999,088
|
1,332,213
|
168,391
|
76,687
|
22,343
|
Number Of Bases
|
902,643,158
|
409,580,444
|
205,675,195
|
141,488,989
|
65,824,918
|
Avg. Contig Size
|
75
|
307
|
1,221
|
1,845
|
2,946
|
N50 Contig Size
|
83
|
505
|
1,438
|
1,910
|
2,854
|
N80 Contig Size
|
38
|
156
|
791
|
1,306
|
2,273
|
N90 Contig Size
|
35
|
122
|
633
|
1,143
|
2,132
|
largest Contig Size
|
19,553
|
19,553
|
19,553
|
19,553
|
19,553
|
- Illumina RNA Sequencing °á°ú
Total number of raw reads
|
- Number of sequences
|
59,658,056
|
- Number of bases
|
8,368,892,629
|
Contig information
|
- Total number of contig
|
167,098
|
- Number of bases
|
178,420,127
|
- Mean length of contig (bp)
|
1,067.8
|
- N50 length of contig (bp)
|
2,097
|
- GC % of contig
|
40.80
|
- Largest contig (bp)
|
27,439
|
- No. of large contigs (¡Ã500bp)
|
81,280
|
Transdecoder information
|
- Total number of contig
|
103,523
|
- Number of bases
|
211,950,910
|
- Mean length of contig (bp)
|
2,047.4
|
- N50 length of contig (bp)
|
3,218
|
- GC % of contig
|
42.60
|
- Largest contig (bp)
|
27,439
|
- No. of large contigs (¡Ã500bp)
|
82,287
|
Unigene information
|
- Total number of unigenes
|
26,991
|
- Number of bases
|
65,644,094
|
- Mean length of unigene (bp)
|
2,432.1
|
- N50 length of unigene (bp)
|
3,358
|
- GC % of unigene
|
42.36
|
- Length ranges (bp)
|
224 – 30,454
|
2) ÇöÀç »óȲ¿¡ ´ëÇÑ °íÂû
°¡) ´ë»óÁ¾ ¹× ½Ã·áÈ®º¸ »óȲ
(1) Ãʱ⠿¹ºñÁ¾À» Æ÷ÇÔÇÑ Ã¤Áý ´ë»óÁ¾ : 23Á¾(ÀýÁöµ¿¹°(°ïÃæ) 9Á¾, ¿¬Ã¼µ¿¹° 8Á¾, ¾ç¼·ù 6Á¾)
(2) äÁý ¿Ï·á Á¾ : 9Á¾(ÀýÁöµ¿¹°(°ïÃæ) 5Á¾, ¿¬Ã¼µ¿¹° 1Á¾, ¾ç¼·ù 3Á¾)
³ª) ¸êÁ¾À§±âµ¿¹°ÀÇ Genome survey
(1) Library QC data
- DNA Library QC
Delivery name
|
Tape
Concentration
(ng/ul)
|
Volume
(ul)
|
Tape Quantity
(ug)
|
Main peak Size
(bp)
|
Result
|
µÎÁ¡¹ÚÀ̻罿¹ú·¹
|
80.559
|
23
|
1.853
|
470
|
pass
|
- RNA Library QC
Delivery name
|
Tape
Concentration
(ng/ul)
|
Volume
(ul)
|
Tape Quantity
(ug)
|
Main peak Size
(bp)
|
Result
|
µÎÁ¡¹ÚÀ̻罿¹ú·¹
|
377.058
|
30
|
11.312
|
280
|
pass
|
(2) °íǰÁú genomic DNA äÃë ¹× Ç¥º»È®º¸
- °Ë¼ö¿ë DNA´Â ´ëºÎºÐ ¸¸Á·ÇÏ¿´°í, ¸êÁ¾À§±âÁ¾ÀÎ ´ë»óÁ¾µéÀº äÁý·®ÀÇ Á¦ÇÑÀ¸·Î ÀÎÇØ È®ÁõÇ¥º»À¸·Î Á¦Ãâ °¡´ÉÇÑ Ç¥º»À» È®º¸ÇÏÁö ¸øÇÏ¿© »çÁøÀ¸·Î ´ëüÇϰíÀÚ ÇÔ.
°Ë¼ö¿ë »ùÇà DNA ³óµµ ¹× ÃÑ ¾ç ÇöȲǥ
|
¡¡Sample ¸í
|
³óµµ(ng/ul)
|
Volume(ul)
|
DNA ÃÑ·®(ug)
|
O.D A260/280
|
ÃßÃâÀÏÀÚ
|
ÀúÀåÀå¼Ò
|
µÎÁ¡¹ÚÀ̻罿¹ú·¹
|
733
|
300
|
219.9
|
2.41
|
170927
|
1XTE pH8.0
|
(3) ±âŸºÐ¼®
A. Genome Size estimation – Jellyfish ÇÁ·Î±×·¥
°¢ ´ë»óÁ¾À» ´ë»óÀ¸·Î Jellyfish ÇÁ·Î±×·¥À¸·Î Genome size¸¦ ¿¹ÃøÇÑ °á°ú´Â ¾Æ·¡¿Í °°´Ù. °¢ ºÐ¼®Àº k-mer °ª 17mer, 19mer, 21mer¸¦ ´ë»óÀ¸·Î ÇÏ¿´°í ±×°ÍÀ» ÅëÇØ ¿¹ÃøµÈ genomeÀÇ Å©±â´Â Estimation genome size ¿¡ Ç¥½ÃµÇ¾ú°í, À̸¦ ÅëÇØ À̹ø »ç¾÷¿¡¼ ºÐ¼®ÇÑ ¼¿ÀÇ ¾çÀÇ coverage´Â Genome coverage depth(Coverage Depth)¿¡ Ç¥±âµÇ¾ú´Ù. ±×¸®°í °¢ Á¾ÀÇ Ç¥ ¾Æ·¡ ±×·¡ÇÁ´Â kmer°ª º°·Î ³ªÅ¸³ depthÀÇ ¿¹Ãø ±×·¡ÇÁ·Î peak°ªÀ» È®ÀÎÇÒ ¼ö ÀÖ´Ù.
»ùÇøí
|
K-mer
(K-mersize)
|
Estimateddepth
(K-merCoveragedepth)
|
Genomecoveragedepth
(Coveragedepth)
|
Estimatedgenome
size
|
µÎÁ¡¹ÚÀ̻罿¹ú·¹
|
17
|
43
|
24.61
|
623,437,265.5
|
19
|
43
|
23.84
|
643,448,832
|
21
|
42
|
24.21
|
633,772,909.7
|

B. ¹Ýº¹¼¿
- ½ÃÄö½ÌÀÌ ¿Ï·áµÈ »ùÇà DNA ÀÇ contigs Áß 1kb ÀÌ»óÀÇ contigsÀÇ ¹Ýº¹¼¿À» Ž»ö (Repeat masking results of DNA contigs)
µÎÁ¡¹ÚÀ̻罿¹ú·¹ (Prosopocoilus blanchardi)
|
|
µÎÁ¡¹ÚÀ̻罿¹ú·¹ (Prosopocoilus blanchardi) ´Â 1kb ÀÌ»óÀÇ contig ÀÇ ¹Ýº¹¼¿Àº 26,991°³ read (65,644,094 bp) À̰í, GC level Àº 42.36%ÀÌ´Ù. Repeat Masking À¸·Î ³ª¿Â ¹Ýº¹¼¿Àº 645,606 bp (ÀüüÀÇ 0.98%) ¸¸Å ÀÖ´Ù. Small RNA Àº 31°³ read (10,221 bp) °¡ ÀÖ°í, Simple repeat ´Â 13,330°³ read (493.923 bp) °¡ ÀÖ´Ù.
|
sequences: 26,991
|
|
total length: 65,644,094 bp (65,644,094 bp excl N/X-runs)
|
|
GC level: 42.36 %
|
|
bases masked: 645,606 bp ( 0.98%)
|
|
¡¡
|
number of
elements
|
length
occupied
|
percentage
of sequence
|
|
|
SINEs:
|
4
|
156 bp
|
0.00%
|
|
¡¡
|
ALUs
|
0
|
0 bp
|
0.00%
|
|
¡¡
|
MIRs
|
0
|
0 bp
|
0.00%
|
|
LINEs:
|
48
|
3,689 bp
|
0.01%
|
|
¡¡
|
LINE1
|
1
|
34 bp
|
0.00%
|
|
¡¡
|
LINE2
|
13
|
945 bp
|
0.00%
|
|
¡¡
|
L3/CR1
|
27
|
2,229 bp
|
0.00%
|
|
LTRelements:
|
58
|
16,338 bp
|
0.02%
|
|
¡¡
|
ERVL
|
0
|
0 bp
|
0.00%
|
|
¡¡
|
ERVL-MaLRs
|
0
|
0 bp
|
0.00%
|
|
¡¡
|
ERV_classI
|
3
|
210 bp
|
0.00%
|
|
¡¡
|
ERV_classII
|
2
|
119 bp
|
0.00%
|
|
DNAelements:
|
89
|
10,543 bp
|
0.02%
|
|
¡¡
|
hAT-Charlie
|
8
|
872 bp
|
0.00%
|
|
¡¡
|
TcMar-Tigger
|
7
|
1,508 bp
|
0.00%
|
|
Unclassified:
|
1
|
66 bp
|
0.00%
|
|
Total interspersed repeats:
|
|
30,792 bp
|
0.05%
|
|
Small RNA:
|
31
|
10,221 bp
|
0.02%
|
|
Satellites:
|
1
|
0 bp
|
0.00%
|
|
Simple repeats:
|
13,330
|
493.923 bp
|
0.75%
|
|

µÎÁ¡¹ÚÀ̻罿¹ú·¹´Â Reference·Î 21,628 bp Å©±âÀÇ Prosopocoilus astacoides blanchardi ¹ÌÅäÄܵ帮¾Æ genomeÀ» ÀÌ¿ëÇÏ¿© ¸ÊÇÎÇÏ¿´´Ù. °¸Àº 396 bp ·Î, Coverage ´Â 98.17%·Î È®ÀεǾú´Ù.
D. Transcriptome data ºÐ¼®
- µÎÁ¡¹ÚÀ̻罿¹ú·¹

µÎÁ¡¹ÚÀ̻罿¹ú·¹ÀÇ KOG °á°ú, ¡°Infomation Storage and Processing¡± ºÎºÐ¿¡¼´Â transcription °ü·ÃÀÌ 5.0%·Î °¡Àå ¸¹ÀÌ ¸ÅĪµÇ°í, ¡°Cellular Processes and Signaling¡± ¿¡¼´Â signal transduction mechanisms (9.8%), post translational modification (6.1%) ·Î ¸¹ÀÌ ¸ÅĪµÈ´Ù. ¡°Metabolism¡± ºÎºÐÀº Æò±Õ 1% ´ë·Î ³·°Ô ¸ÅĪµÇ°í, Multiple Function Àº 20.3% ÀÌ´Ù.

µÎÁ¡¹ÚÀ̻罿¹ú·¹ÀÇ GO °á°ú, Biological Process¿¡¼ membrane (1,713°³), cell (1,601°³) ¼øÀ¸·Î ¸¹ÀÌ ¸ÅĪµÈ´Ù. Molecular Function ¿¡¼´Â binding (2,981°³), catalytic activity (2,516°³) ¼øÀ¸·Î °¡Àå ¸¹ÀÌ ¸ÅĪµÈ´Ù. ¶ÇÇÑ, Cellular Component ºÎºÐ¿¡¼´Â cellular process (2,429°³), metabolic process (2,408°³), single-organism process (1,445°³) ¼øÀ¸·Î ¸¹ÀÌ ¸ÅĪµÈ´Ù.
(4) Microsatellite È帱º
A. SSR statistics
µÎÁ¡¹ÚÀ̻罿¹ú·¹
|
Repeats
|
4
|
5
|
6
|
7
|
8
|
9
|
10
|
11
|
12
|
13
|
¡¦
|
Total
|
Di
|
0
|
0
|
374
|
230
|
102
|
96
|
40
|
21
|
2
|
0
|
|
869
|
Tri
|
0
|
1,947
|
907
|
291
|
168
|
14
|
11
|
2
|
7
|
0
|
|
3,357
|
Tetra
|
677
|
225
|
74
|
2
|
1
|
0
|
0
|
0
|
0
|
0
|
|
979
|
Penta
|
117
|
18
|
7
|
0
|
0
|
0
|
0
|
0
|
0
|
0
|
|
142
|
Hexa
|
66
|
2
|
0
|
0
|
0
|
0
|
0
|
0
|
0
|
0
|
|
68
|
Total
|
860
|
2,192
|
1,362
|
523
|
271
|
110
|
51
|
23
|
9
|
0
|
|
5,415
|
¡æ SSR °Ë»ö°á°ú ÃÑ 5,415°³ÀÇ SSR ¼¿À» È®ÀÎÇÒ ¼ö ÀÖ¾úÀ¸¸ç, Tri motif °¡ 3,357°³·Î °¡Àå ¸¹ÀÌ Á¸ÀçÇÏ´Â °ÍÀ» È®ÀÎ ÇÒ ¼ö ÀÖ¾úÀ¸¸ç, À̸¦ ±â¹ÝÀ¸·Î ÇÏ¿© SSR marker È帱º¿¡ ´ëÇÑ primer ¸¦ 7,942°³¸¦ µðÀÚÀÎ ÇÏ¿´´Ù.
B. Primer sequence
¡æ °¢ Á¾ÀÇ À¯ÀüÀÚ¸¦ ºÐ¼®ÇÏ¿© ±× Á¾À» ±¸º°ÇÒ ¼ö ÀÖ´Â primer¸¦ ¸¸µç´Ù. primer ÀÇ Á¶°ÇÀº Motif ¾à 4~6°³À̸ç, Forward ¿Í Reverse primer °¡ 18~22°³ Á¤µµÀ̰í Tm°ªÀÌ 54.5~55.5 Á¤µµ°¡ Àû´çÇÏ´Ù.
- µÎÁ¡¹ÚÀ̻罿¹ú·¹
Sequence
|
Motif
|
Forward Primer
|
Tm
|
Reverse Primer
|
Pb_Uni_00010
|
(AACT)6
|
GGAGCACCTCATTTTAAGTATC
|
54.79
|
CAAGACACTCTCTTCAGACCA
|
55.52
|
Pb_Uni_00250
|
(CCGAAA)4
|
GGTTGCACTCAGAGTTAGTGT
|
54.5
|
CGATGCTACATAACAATTCCT
|
54.56
|
Pb_Uni_00474
|
(CTT)10
|
TTCAGGGGTGACTTTTAAGTAT
|
54.73
|
TGTTCATCGAAATTTAAGTGG
|
55.41
|
Pb_Uni_00540
|
(AAG)8
|
AGCAATTAGGCCAAACATT
|
54.91
|
CATGTTTCGTCGTAAAGTCAT
|
55.34
|
Pb_Uni_02176
|
(CCATTC)4
|
TTCAAATACCTCAGAGTGACC
|
54.34
|
CTTCGTGTGTACCGTACTGAT
|
55.29
|
Pb_Uni_02200
|
(TTG)8
|
CAATAGACCGATATTTTGCAC
|
54.96
|
ATACAAAAACACGGCTAACAA
|
55.12
|
Pb_Uni_02211
|
(TAAAA)5
|
GACGACATCCATTTAGAAAAA
|
54.49
|
GACGCAAGCAAATCCTAATA
|
55.66
|
Pb_Uni_04330
|
(TTC)9
|
TATAAGTCTCGGGGTACTCGT
|
55.54
|
TCAAATGACGAATCAGAAAAC
|
55.36
|
Pb_Uni_04333
|
(GAA)9
|
AAGAACCCTACGAATCTTCC
|
54.97
|
TATAAGTCTCGGGGTACTCGT
|
55.54
|
Pb_Uni_08167
|
(TAA)9
|
TTCACTTTGTTTAAGGACGAG
|
54.8
|
AAATGTCGTGTGTTCAATTTC
|
55.13
|
Pb_Uni_08168
|
(TTA)9
|
AAATGTCGTGTGTTCAATTTC
|
55.13
|
TTGTTTAAGGACGAGCAACT
|
55.21
|
Pb_Uni_10329
|
(TGC)9
|
TGCTGCTGCTGCTGTTGT
|
60.52
|
TCAGCAAGTACAAACATTCAA
|
54.51
|
Pb_Uni_13287
|
(TTAAA)5
|
TTGACCGATTTTTATTAGTGC
|
54.71
|
TATTTGACGAATTGGAGGTTA
|
54.94
|
Pb_Uni_17182
|
(CAG)9
|
TCAGCAAGTACAAACATTCAA
|
54.51
|
CTGCTGCTGCTGTTGTTG
|
57.71
|
Pb_Uni_23230
|
(ACAA)7
|
CGCAGAGTATGTATGTATAAAA
|
50.32
|
TTAGCGTTAATTTAGCGAATC
|
54.37
|
¿ÃÇØ 17³âµµ¿¡ äÁýÇÑ 6Á¾ÀÇ À¯ÀüÀÚ¸¦ ºÐ¼®ÇÏ¿© ±× Á¾À» ±¸º°ÇÒ ¼ö ÀÖ´Â Barcode ¼¿À» ¸¸µé¾ú°í, ±× Barcode ¼¿À» °¡Áö°í NCBI (National Center for Biotechnology Information) »çÀÌÆ®¿¡ ÀÖ´Â BLAST (Basic Local Alignment Search Tool)À» ÀÌ¿ëÇÏ¿© °á°ú¸¦ È®ÀÎÇÏ¿´´Ù. °¢ Á¾ÀÇ ¼¿µéÀÇ BLAST °á°ú ¸¶Ä¿·Î¼ ÁÖ·Î »ç¿ëµÇ´Â COI ¼¿°ú ¸ÅÄ¡µÇ´Â °ÍÀ¸·Î º¸¾Æ ¹ÙÄÚµå ¼¿·Î »ç¿ë°¡´ÉÇÒ °ÍÀ¸·Î »ç·áµÈ´Ù.

»ùÇÃÁ¾
|
Number of Contig (>1kb)
|
Number of Bases (>1kb)
|
N50 Contig Size
|
Number of gene prediction
|
µÎÁ¡¹ÚÀ̻罿¹ú·¹
|
20,548
|
61,701,123
|
3,358
|
16,273
|
°¢Á¾º°·Î transcriptome ¼¿À» ´ë»óÀ¸·Î 1kb ÀÌ»ó¸¸À» ¼±ÅÃÇÏ¿© Gene predcition ÇÑ °á°ú À§ÀÇ ¸ÅĪµÈ contig, base ¼ö´Â À§ÀÇ Ç¥¿Í °°°í N50µÇ´Â ContigÀÇ ±æÀ̵µ º¸Åë 1~3kb Á¤µµ·Î ³ªÅ¸³µ´Ù. ±×¸®°í À̵éÀ» ÅëÇØ ¿¹ÃøµÈ À¯ÀüÀÚÀÇ ¼ö´Â 8õ°³¿¡¼ 4¸¸°³ ÀÌ»óÀ¸·Î ´Ù¾çÇÏ°Ô ¿¹ÃøµÇ¾ú´Ù.
¡Ø Long contigÀÇ Gene prediction °á°ú ±×¸² ¿¹½Ã

|