Á¦ÁÖ¹è²ÅÅÐ´ÞÆØÀÌ (Aegista (Plectotropis) quelpartensis)

|
¢Ã ±¹ ¸í: Á¦ÁÖ¹è²ÅÅÐ´ÞÆØÀÌ
¢Ã ÇÐ ¸í: Aegista (Plectotropis) quelpartensis Pilsbry & Hirase
¢Ã ÁöÁ¤¹øÈ£: °íÀ¯Á¾
¢Ã °è Åë: º¹Á·°-ÁøÀ¯Æó¸ñ-´ÞÆØÀ̰ú
|
¡Ý »ý±è»õ : ÆÐ°¢Àº žÀÌ ³ôÁö ¾ÊÀº ³ª¼±»ó ¿ø»Ô¸ð¾çÀ» ÀÌ·é´Ù. ²®ÁúÀº ºñ±³Àû ¾ã°í ¹àÀº °¥»öÀ» ¶ì¸ç ¹«µò ±¤ÅÃÀÌ ³´Ù. ÆÐ°¢ Àü¸é¿¡´Â ¼ºÀå¼±À» µû¶ó °¡´Â °¸ð°¡ ÃÎÃÎÇÏ°Ô ³ªÅ¸³ª¸ç üÃþ ÁÖ¿¬¿¡´Â ´õ¿í ±æ¾îÁ®¼ ºñ´Ã ¸ð¾çÀÇ ±ä °¸ð°¡ üÃþ °¡ÀåÀÚ¸®¸¦ µÎ¸£°í ÀÖ°í üÃþ ¹Ø¸é¿¡µµ °¡´Â °¸ð°¡ ¼ºÀå¼±À» µû¶ó ¿Áö¾î ³ªÅ¸³´Ù. ³ªÃþÀº 7.5Ãþ ÀÌ¸ç ºÀÇÕÀº ±íÁö ¾ÊÀ¸³ª ¶Ñ·ÇÇÏ´Ù. °¢ ³ªÃþÀº Á¡ÁøÀûÀ¸·Î Áõ°¡ÇÏ¸ç ¸¶Áö¸· ³ªÃþÀÇ °¢±¸´Â ¾Æ·¡·Î óÁø´Ù. üÃþ ¾î±ú´Â Çü¼ºµÇÁö ¾Ê°í °¡ÀåÀÚ¸®ÀÇ °¢Àº °ÇÏ¸ç °¢À» µû¶ó ºñ´Ã¸ð¾çÀÇ ±ä °¸ð°¡ ÀÖ´Ù. üÃþ ¹Ø¸éÀº ÆíÆòÇÏÁö ¾Ê°í µÕ±Û´Ù. üÃþ ³ôÀÌ´Â 8.3mm·Î ÆÐ°¢ ³ôÀÌÀÇ 78%Á¤µµ ÀÌ´Ù. °¢±¸´Â Å©°í ÃÊ½Â´Þ ¸ð¾çÀÌ¸ç ½ÉÇÏ°Ô ºñ½ºµëÇÏ´Ù. °¢±¸ °¡ÀåÀÚ¸®´Â ¹é»öÀÌ´Ù. ÆÐ°¢ÀÇ Å©±â´Â ³ôÀÌ 12mm, Áö¸§ 21mmÀÌ´Ù.
¡Ý ¼½ÄÁö : Àΰ¡ ±Ùó µ¹´ãÀ̳ª ¾ß»ê¿¡ ¼½ÄÇÑ´Ù.
¡Ý ºÐÆ÷ : Çѱ¹ °íÀ¯Á¾À¸·Î Á¦ÁÖµµ°¡ ¸ð½Ä»êÁöÀ̸ç Á¦ÁÖµµ, °æ³²¿¡ ºÐÆ÷ÇÑ´Ù.
1) äÁýÁ¤º¸
- äÁýÇã°¡: °íÀ¯Á¾
- ä Áý ÀÚ: °¿ø´ëÇб³ ÀÌÁØ»ó
2) äÁýÇöȲ ¹× »ùÇÃó¸®
Á¦ÁÖ¹è²ÅÅÐ´ÞÆØÀÌ Ã¤ÁýÇöȲ
|
»ùÇÃó¸®
|
äÁýÀÏ
|
°³Ã¼ ¼ö
|
DNAseq
|
RNAseq
|
Ç¥º»
|
2014.08.26
|
14 EA
|
13 EA (¹ß)
|
13 EA (³»Àå)
|
1 EA
|
3) QC Data
»ùÇÃÁ¾
|
DNA QC
|
RNA QC
|
Result
|
Concentration (ug/ul)
|
Total Conc. (ug)
|
Concentration (ug/ul)
|
RIN (>7)
|
Á¦ÁÖ¹è²ÅÅÐ´ÞÆØÀÌ
|
162
|
17.8
|
393
|
9.5
|
pass
|
4) ±âÁ¸ NCBI Taxonomy À¯ÀüÁ¤º¸ ÇöȲ
À¯ÀüÁ¤º¸ Á¾·ù
|
Aegista ¼Ó À¯ÀüÀÚ¿ø
|
Aegista (Plectotropis) quelpartensis
À¯ÀüÀÚ¿ø
|
Nucleotide
|
359
|
-
|
Protein
|
251
|
-
|
Genome
|
2
|
-
|
Gene
|
74
|
-
|
5) Sequencing ºÐ¼® °á°ú
- Illumina DNA Sequncing °á°ú
Input information
|
¡¡
|
Region1
|
Region2
|
Region3
|
Region4
|
Total
|
raw data
|
Number Of Reads
|
101,887,398
|
101,887,398
|
17,581,833
|
17,581,833
|
238,938,462
|
Number Of Bases
|
12,837,812,148
|
12,837,812,148
|
2,215,310,958
|
2,215,310,958
|
30,106,246,212
|
error correction
|
Number Of Reads
|
97,446,876
|
95,671,796
|
8,807,996
|
8,464,392
|
210,391,060
|
Number Of Bases
|
11,085,272,433
|
10,725,990,135
|
870,953,258
|
825,183,376
|
23,507,399,202
|
pair reads
|
Number Of Reads
|
|
184,381,992
|
|
12,007,658
|
196,389,650
|
Number Of Bases
|
|
20,914,197,839
|
|
1,240,872,266
|
22,155,070,105
|
single reads
|
Number Of Reads
|
|
8,736,680
|
|
5,264,730
|
14,001,410
|
Number Of Bases
|
|
897,064,729
|
|
455,264,368
|
1,352,329,097
|
Assembly Results
|
Scaffold Metrics
|
> 1 bp*
|
> 100 bp
|
> 500 bp**
|
> 1000 bp
|
> 2000 bp
|
Number Of Scaffolds
|
2,277,010
|
2,277,010
|
519,523
|
159,152
|
19,123
|
Number Of Bases
|
859,264,857
|
859,264,857
|
484,643,590
|
233,650,081
|
47,958,340
|
Avg. Scaffold Size
|
377
|
377
|
932
|
1,468
|
2,507
|
N50 Scaffold Size
|
587
|
587
|
976
|
1,443
|
2,421
|
N80 Scaffold Size
|
231
|
231
|
659
|
1,143
|
2,135
|
N90 Scaffold Size
|
152
|
152
|
576
|
1,068
|
2,063
|
largest Scaffold Size
|
9,274
|
9,274
|
9,274
|
9,274
|
9,274
|
Contig Metrics
|
> 1 bp*
|
> 100 bp
|
> 500 bp**
|
> 1000 bp
|
> 2000 bp
|
Number Of Contigs
|
21,566,578
|
3,882,699
|
245,566
|
25,337
|
621
|
Number Of Bases
|
1,924,868,632
|
927,402,447
|
176,337,472
|
31,720,840
|
1,463,686
|
Avg. Contig Size
|
89
|
238
|
718
|
1,251
|
2,356
|
N50 Contig Size
|
91
|
261
|
703
|
1,207
|
2,262
|
N80 Contig Size
|
49
|
160
|
564
|
1,065
|
2,094
|
N90 Contig Size
|
47
|
137
|
529
|
1,030
|
2,046
|
Largest Contig Size
|
8,119
|
8,119
|
8,119
|
8,119
|
8,119
|
- Illumina RNA Sequncing °á°ú
Input information
|
¡¡
|
Region1
|
Region2
|
Region3
|
Region4
|
Total
|
raw data
|
Number Of Reads
|
101,851,586
|
101,851,586
|
17,769,443
|
17,769,443
|
239,242,058
|
Number Of Bases
|
12,833,299,836
|
12,833,299,836
|
2,238,949,818
|
2,238,949,818
|
30,144,499,308
|
error correction
|
Number Of Reads
|
100,576,427
|
100,081,232
|
16,991,601
|
16,829,182
|
234,478,442
|
Number Of Bases
|
12,440,237,553
|
12,420,785,646
|
2,069,164,472
|
2,044,321,924
|
28,974,509,595
|
pair reads
|
Number Of Reads
|
|
199,237,908
|
|
33,153,936
|
232,391,844
|
Number Of Bases
|
|
24,713,305,393
|
|
4,049,130,552
|
28,762,435,945
|
single reads
|
Number Of Reads
|
|
1,419,751
|
|
666,847
|
2,086,598
|
Number Of Bases
|
|
147,717,806
|
|
64,355,844
|
212,073,650
|
Assembly Results
|
Scaffold Metrics
|
> 1 bp*
|
> 100 bp
|
> 500 bp**
|
> 1000 bp
|
> 2000 bp
|
Number Of Scaffolds
|
319,952
|
319,952
|
39,978
|
12,054
|
2,597
|
Number Of Bases
|
93,918,909
|
93,918,909
|
39,476,731
|
20,394,949
|
7,723,326
|
Avg. Scaffold Size
|
293
|
293
|
987
|
1,691
|
2,973
|
N50 Scaffold Size
|
392
|
392
|
1,028
|
1,676
|
2,903
|
N80 Scaffold Size
|
164
|
164
|
652
|
1,199
|
2,267
|
N90 Scaffold Size
|
131
|
131
|
570
|
1,094
|
2,125
|
largest Scaffold Size
|
17,321
|
17,321
|
17,321
|
17,321
|
17,321
|
Contig Metrics
|
> 1 bp*
|
> 100 bp
|
> 500 bp**
|
> 1000 bp
|
> 2000 bp
|
Number Of Contigs
|
2,954,097
|
437,327
|
27,246
|
4,975
|
614
|
Number Of Bases
|
254,535,026
|
98,293,159
|
22,112,723
|
7,339,704
|
1,651,717
|
Avg. Contig Size
|
86
|
224
|
811
|
1,475
|
2,690
|
N50 Contig Size
|
91
|
242
|
790
|
1,421
|
2,580
|
N80 Contig Size
|
52
|
143
|
586
|
1,130
|
2,162
|
N90 Contig Size
|
47
|
120
|
540
|
1,059
|
2,095
|
Largest Contig Size
|
8,859
|
8,859
|
8,859
|
8,859
|
8,859
|
³ª) ¸êÁ¾À§±âÁ¾ µ¿¹° Genome Survey
°Ë¼ö¿ë »ùÇà DNA ³óµµ ¹× ÃÑ ¾ç, È®ÁõÇ¥º» ÇöȲǥ
|
¡¡Sample¸í
|
³óµµ(ng/ul)
|
Volume(ul)
|
DNA ÃÑ·®(ug)
|
O.D A260/280
|
È®ÁõÇ¥º»
|
äÁýÇã°¡ÀÏ
|
äÁýÀÏ
|
Á¦ÁÖ¹è²ÅÅÐ´ÞÆØÀÌ
|
104
|
996
|
103
|
1.72
|
¡Û
|
°íÀ¯Á¾
|
2014.8.26
|
(2) À¯Àüü ¼¿È®º¸ : ÇöÀç 20Á¾ È®º¸
Sample
|
±¸ºÐ
|
raw data size
|
trim data size
|
ºñ°í
|
Á¦ÁÖ¹è²ÅÅÐ´ÞÆØÀÌ
|
DNA
|
30,106,246,212
|
23,507,399,202
|
¡¡
|
RNA
|
30,144,499,308
|
28,974,509,595
|
¡¡
|
(3) ±âŸºÐ¼®
A. Genome Size estimation
»ù Çà ¸í
|
Kmer size
|
Kmer total
|
Pk depth
|
Genome_size
|
Coverage(X)
|
Á¦ÁÖ¹è²ÅÅÐ´ÞÆØÀÌ
|
23
|
15,894,434,088
|
147
|
108,125,401
|
188.462
|
B. ¹Ýº¹¼¿
- ½ÃÄö½ÌÀÌ ¿Ï·áµÈ Á¦ÁÖ¹è²ÅÅÐ´ÞÆØÀÌ DNA ¹× RNAÀÇ contigs Áß 1kb ÀÌ»óÀÇ contigsÀÇ ¹Ýº¹¼¿À» Ž»ö(Left; Repeat masking results of DNA contigs, Right; Repeat masking results of RNA contigs).
sequences: 26,032
|
|
sequences: 7,108
|
total length: 32,608,921 bp (32,608,921 bp excl N/X-runs)
|
|
total length: 11,220,308 bp (11,220,308 bp excl N/X-runs)
|
GC level: 40.72%
|
|
GC level: 44.87%
|
bases masked: 54749 bp (0.17%)
|
|
bases masked: 7823 bp (0.07%)
|
|
number of
elements
|
length
occupied
|
percentage
of sequence
|
|
|
number of
elements
|
length
occupied
|
percentage
of sequence
|
SINEs:
|
5
|
229 bp
|
0.00%
|
|
SINEs:
|
0
|
0 bp
|
0.00%
|
|
ALUs
|
0
|
0 bp
|
0.00%
|
|
|
ALUs
|
0
|
0 bp
|
0.00%
|
|
MIRs
|
2
|
95 bp
|
0.00%
|
|
|
MIRs
|
0
|
0 bp
|
0.00%
|
LINEs:
|
105
|
25,847 bp
|
0.08%
|
|
LINEs:
|
12
|
1,931 bp
|
0.02%
|
|
LINE1
|
7
|
395 bp
|
0.00%
|
|
|
LINE1
|
2
|
87 bp
|
0.00%
|
|
LINE2
|
3
|
134 bp
|
0.00%
|
|
|
LINE2
|
1
|
115 bp
|
0.00%
|
|
L3/CR1
|
14
|
879 bp
|
0.00%
|
|
|
L3/CR1
|
3
|
160 bp
|
0.00%
|
LTR elements:
|
17
|
2,171 bp
|
0.01%
|
|
LTR elements:
|
4
|
577 bp
|
0.01%
|
|
ERVL
|
0
|
0 bp
|
0.00%
|
|
|
ERVL
|
0
|
0 bp
|
0.00%
|
|
ERVL-MaLRs
|
0
|
0 bp
|
0.00%
|
|
|
ERVL-MaLRs
|
1
|
66 bp
|
0.00%
|
|
ERV_classI
|
13
|
1,802 bp
|
0.00%
|
|
|
ERV_classI
|
2
|
203 bp
|
0.00%
|
|
ERV_classII
|
1
|
39 bp
|
0.00%
|
|
|
ERV_classII
|
0
|
0 bp
|
0.00%
|
DNA elements:
|
179
|
20,437 bp
|
0.06%
|
|
DNA elements:
|
28
|
4,899 bp
|
0.04%
|
|
hAT-Charlie
|
61
|
7,320 bp
|
0.02%
|
|
|
hAT-Charlie
|
15
|
3,017 bp
|
0.03%
|
|
TcMar-Tigger
|
29
|
3,092 bp
|
0.01%
|
|
|
TcMar-Tigger
|
2
|
105 bp
|
0.00%
|
Unclassified:
|
2
|
102 bp
|
0.00%
|
|
Unclassified:
|
0
|
0 bp
|
0.00%
|
Total interspersed repeats:
|
|
48,786 bp
|
0.15%
|
|
Total interspersed repeats:
|
|
7,407 bp
|
0.07%
|
Small RNA:
|
52
|
5,963 bp
|
0.02%
|
|
Small RNA:
|
2
|
416 bp
|
0.00%
|
Satellites:
|
0
|
0 bp
|
0.00%
|
|
Satellites:
|
0
|
0 bp
|
0.00%
|
Simple repeats:
|
0
|
0 bp
|
0.00%
|
|
Simple repeats:
|
0
|
0 bp
|
0.00%
|
Low complexity:
|
0
|
0 bp
|
0.00%
|
|
Low complexity:
|
0
|
0 bp
|
0.00%
|
D. Mito-genome Map

(4) Microsatellite È帱º
A. SSR statistics
Motif(-mer)
|
2
|
3
|
4
|
5
|
6
|
7
|
8
|
9
|
10
|
Total
|
Á¦ÁÖ¹è²ÅÅÐ´ÞÆØÀÌ
|
4,423
|
1,950
|
566
|
11
|
3
|
1
|
2
|
1
|
-
|
6,957
|
B. ¹ÙÄڵ弿
>Á¦ÁÖ¹è²ÅÅÐ´ÞÆØÀÌ(Aegista quelpartensis)
TTATATATATTATTTGGTGTATGATGTGGTATAGTAGGATCTGGTTTATCAATATTAATTCGTATAGAACTAGGGACTTGTAATGTATTAAGGGATAGTCATTTTTTTAATGTAATCGTTACAGCTCATGCATTTGTAATAATTTTTTTTATAGTAATACCTATTATAATTGGAGGTTTTGGAAATTGAATAGTTCCTTTATTAATTGGAGCTCCTGATATAAGTTTTCCACGAATAAATAATATAAGCTTTTGATTATTACCTCCTTCATTTATTTTATTAATTTCTAGAAGGTTAGTTGAGGGTGGAGCTGGTACTGGATGAACAGTATATCCACCTTTAAGTTCTATTGTAGGTCATAGAGGAGCCTCAGTAGATTTAGCCATTTTTTCTCTGCATTTGGCTGGTATATCTTCAATTCTAGGTGCTATTAATTTTATTACAACAATTTTTAATATGCGTAGTCCAGGAATTTCATTAGAACGAATAAGTTTATTTGTATGATCTATTTTAATTACTGTATTTTTATTACTTTTATCTTTACCTGTTTTAGCAGGTGCTATTACTATATTGCTTACTGATCGAAATTTTAATACTTCTTTCTTTGATCCTGCAGGCGGAGGAGATCCAATTTTATATCAGCATTT
Lozekia deubeli : family ºÎÅÍ ´Ù¸§
Baudinella thielei :¡¡superfamily ºÎÅÍ ´Ù¸§

|