Á¦ÁÖ¹è²Å´ÞÆØÀÌ (Aegista (Aegista) chejuensis)

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¢Ã ±¹ ¸í: Á¦ÁÖ¹è²Å´ÞÆØÀÌ
¢Ã ÇÐ ¸í: Aegista chejuensis Pilsbry & Hirase
¢Ã ÁöÁ¤¹øÈ£: °íÀ¯Á¾
¢Ã °è Åë: º¹Á·°-ÁøÀ¯Æó¸ñ-´ÞÆØÀ̰ú
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¡Ý »ý±è»õ : ÆÐ°¢Àº ³ªÅ¾ÀÌ ³·Áö ¾ÊÀº ³ª¼±»óÀÇ ¿ø»Ô¸ð¾çÀÌ¸ç ²®ÁúÀº ºñ±³Àû ´Ü´ÜÇÏ´Ù. ÆÐ°¢ Àü¸é¿¡´Â ±¤ÅÃÀÌ ³ª´Â ¿¬ÇÑ °¥»öÀÇ °¢ÇÇ·Î µ¤¿© ÀÖ°í, ¸Å¿ì °¡´Â ½Ç¸ð¾çÀÇ ±¼°îÀÌ ¼ºÀå¼±À» µû¶ó ³ª¼±»óÀ¸·Î Á¶¹ÐÇÏ°Ô »¸¾î ÀÖ´Ù. ¹Ø¸éÀº °¡´Ã°í ÃÎÃÎÇÑ ¼ºÀå¼±ÀÌ ³ªÅ¸³ª°í ÀÖÀ¸¸ç ±¼°îÀÌ ½ÉÇÏÁö ¾Ê¾Æ¼ ÆÐ°¢ Àü¸éº¸´Ù ¸Å²ô·´´Ù. Å©±â´Â ³ôÀÌ 6.2mm, Áö¸§ 10.2mm´Ù. ³ªÃþÀº 6~6.5ÃþÀ̸ç Ȳ°¥»öÀ» ¶í´Ù. °¢ ³ªÃþÀÇ Å©±â´Â ±ÔÄ¢ÀûÀ¸·Î Á¶±Ý¾¿ Áõ°¡ÇÑ´Ù. ¸¶Áö¸· ³ªÃþÀÇ °¢±¸´Â ¾Æ·¡·Î óÁöÁö ¾ÊÀ¸¸ç üÃþ °¡ÀåÀÚ¸®´Â °¢ÀÌ ¾ø¾î µÕ±Û´Ù. °¢±¸´Â ÆÐ°¢¿¡ ºñÇÏ¿© ºñ±³Àû Å©°í ºñ½ºµëÇÏ¸ç µÕ±Ù Ãʽ´޸ð¾çÀÌ´Ù.
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¡Ý ºÐÆ÷ : Çѱ¹ °íÀ¯Á¾À¸·Î ¸ð½Ä»êÁö´Â Á¦ÁÖµµÀ̸ç Áøµµ, ³²Çؾȿ¡ ºÐÆ÷ÇÑ´Ù.
1) äÁýÁ¤º¸
- äÁýÇã°¡: °íÀ¯Á¾
- ä Áý ÀÚ: °¿ø´ëÇб³ ÀÌÁØ»ó
2) äÁýÇöȲ ¹× »ùÇÃó¸®
Á¦ÁÖ¹è²Å´ÞÆØÀÌ Ã¤ÁýÇöȲ
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»ùÇÃó¸®
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äÁýÀÏ
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°³Ã¼ ¼ö
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DNAseq
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RNAseq
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Ç¥º»
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2014.08.26
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9 EA
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4 EA
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4 EA
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1 EA
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3) QC Data
»ùÇÃÁ¾
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DNA QC
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RNA QC
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Result
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Concentration (ug/ul)
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Total Conc. (ug)
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Concentration (ug/ul)
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RIN (>7)
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Á¦ÁÖ¹è²Å´ÞÆØÀÌ
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113
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9
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254
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9.8
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pass
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4) ±âÁ¸ NCBI Taxonomy À¯ÀüÁ¤º¸ ÇöȲ
À¯ÀüÁ¤º¸ Á¾·ù
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Aegista ¼Ó À¯ÀüÀÚ¿ø
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Aegista (Aegista) chejuensis
À¯ÀüÀÚ¿ø
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Nucleotide
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359
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-
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Protein
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251
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-
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Genome
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2
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-
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Gene
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74
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-
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5) Sequencing ºÐ¼® °á°ú
- Illumina DNA Sequncing °á°ú
Input information
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¡¡
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Region1
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Region2
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Region3
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Region4
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Total
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raw data
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Number Of Reads
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118,506,288
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118,506,288
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10,275,102
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10,275,102
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257,562,780
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Number Of Bases
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14,931,792,288
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14,931,792,288
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1,294,662,852
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1,294,662,852
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32,452,910,280
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error correction
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Number Of Reads
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109,658,743
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108,690,436
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4,491,029
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4,422,673
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227,262,881
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Number Of Bases
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12,563,157,322
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12,348,029,788
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456,363,875
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444,403,694
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25,811,954,679
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pair reads
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Number Of Reads
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209,527,066
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6,725,334
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216,252,400
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Number Of Bases
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24,031,628,914
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713,837,970
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24,745,466,884
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single reads
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Number Of Reads
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8,822,113
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2,188,368
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11,010,481
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Number Of Bases
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879,558,196
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186,929,599
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1,066,487,795
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Assembly Results
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Scaffold Metrics
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> 1 bp*
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> 100 bp
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> 500 bp**
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> 1000 bp
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> 2000 bp
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Number Of Scaffolds
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2,215,276
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2,215,276
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511,727
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186,167
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37,418
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Number Of Bases
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886,619,538
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886,619,538
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540,436,120
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313,208,676
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111,951,531
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Avg. Scaffold Size
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400
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400
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1,056
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1,682
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2,991
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N50 Scaffold Size
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680
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680
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1,137
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1,659
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2,791
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N80 Scaffold Size
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240
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240
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703
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1,208
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2,238
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N90 Scaffold Size
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148
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148
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595
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1,097
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2,111
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largest Scaffold Size
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99,405
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99,405
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99,405
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99,405
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99,405
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Contig Metrics
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> 1 bp*
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> 100 bp
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> 500 bp**
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> 1000 bp
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> 2000 bp
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Number Of Contigs
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21,540,436
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4,140,248
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258,618
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29,414
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2,596
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Number Of Bases
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1,961,131,938
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968,202,411
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195,406,419
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45,440,146
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12,182,373
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Avg. Contig Size
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91
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233
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755
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1,544
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4,692
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N50 Contig Size
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96
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249
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717
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1,347
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5,704
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N80 Contig Size
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50
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155
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568
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1,096
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2,685
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N90 Contig Size
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47
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134
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532
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1,045
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2,240
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Largest Contig Size
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70,769
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70,769
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70,769
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70,769
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70,769
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- Illumina RNA Sequncing °á°ú
Input information
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¡¡
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¡¡
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¡¡
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Region1
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Region2
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Total
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raw data
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Number Of Reads
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128,327,935
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128,327,935
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256,655,870
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Number Of Bases
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16,169,319,810
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16,169,319,810
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32,338,639,620
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error correction
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Number Of Reads
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127,109,211
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126,147,475
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253,256,686
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Number Of Bases
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15,739,923,181
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15,660,526,491
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31,400,449,672
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pair reads
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Number Of Reads
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251,314,118
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Number Of Bases
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31,188,107,081
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single reads
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Number Of Reads
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|
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1,942,568
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Number Of Bases
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|
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212,342,591
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Assembly Results
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Scaffold Metrics
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> 1 bp*
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> 100 bp
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> 500 bp**
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> 1000 bp
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> 2000 bp
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Number Of Scaffolds
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228,106
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228,106
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34,622
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11,146
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2,646
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Number Of Bases
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74,648,423
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74,648,423
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35,620,275
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19,569,119
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8,016,049
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Avg. Scaffold Size
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327
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327
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1,028
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1,755
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3,029
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N50 Scaffold Size
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467
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467
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1,096
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1,763
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2,940
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N80 Scaffold Size
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189
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189
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667
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1,233
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2,278
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N90 Scaffold Size
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134
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134
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576
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1,108
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2,128
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largest Scaffold Size
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14,943
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14,943
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14,943
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14,943
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14,943
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Contig Metrics
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> 1 bp*
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> 100 bp
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> 500 bp**
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> 1000 bp
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> 2000 bp
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Number Of Contigs
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3,795,144
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321,887
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22,581
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5,292
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722
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Number Of Bases
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230,294,252
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74,003,080
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19,581,172
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7,973,399
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1,940,159
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Avg. Contig Size
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60
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229
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867
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1,506
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2,687
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N50 Contig Size
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63
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261
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870
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1,472
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2,638
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N80 Contig Size
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35
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138
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607
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1,148
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2,164
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N90 Contig Size
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32
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115
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549
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1,066
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2,077
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Largest Contig Size
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7,490
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7,490
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7,490
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7,490
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7,490
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³ª) ¸êÁ¾À§±âÁ¾ µ¿¹° Genome Survey
°Ë¼ö¿ë »ùÇà DNA ³óµµ ¹× ÃÑ ¾ç, È®ÁõÇ¥º» ÇöȲǥ
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¡¡Sample¸í
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³óµµ(ng/ul)
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Volume(ul)
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DNA ÃÑ·®(ug)
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O.D A260/280
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È®ÁõÇ¥º»
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äÁýÇã°¡ÀÏ
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äÁýÀÏ
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Á¦ÁÖ¹è²Å´ÞÆØÀÌ
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152
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996
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151
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1.54
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¡Û
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°íÀ¯Á¾
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2014.8.26
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(2) À¯Àüü ¼¿È®º¸ : ÇöÀç 20Á¾ È®º¸
Sample
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±¸ºÐ
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raw data size
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trim data size
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ºñ°í
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Á¦ÁÖ¹è²Å´ÞÆØÀÌ
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DNA
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32,452,910,280
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25,811,954,679
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¡¡
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RNA
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32,338,639,620
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31,400,449,672
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¡¡
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(3) ±âŸºÐ¼®
A. Genome Size estimation
»ù Çà ¸í
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Kmer size
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Kmer total
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Pk depth
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Genome_size
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Coverage(X)
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Á¦ÁÖ¹è²Å´ÞÆØÀÌ
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23
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18,486,980,928
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178
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103,859,443
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228.205
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B. ¹Ýº¹¼¿
- ½ÃÄö½ÌÀÌ ¿Ï·áµÈ Á¦ÁÖ¹è²Å´ÞÆØÀÌ DNA ¹× RNAÀÇ contigs Áß 1kb ÀÌ»óÀÇ contigsÀÇ ¹Ýº¹¼¿À» Ž»ö(Left; Repeat masking results of DNA contigs, Right; Repeat masking results of RNA contigs).
sequences: 29,640
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sequences: 5,292
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total length: 45736033 bp (45736033 bp excl N/X-runs)
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total length: 7,973,399 bp (7,973,399 bp excl N/X-runs)
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GC level: 41.78%
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GC level: 45.61%
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bases masked: 60,083 bp (0.13%)
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bases masked: 4079 bp (0.05%)
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number of
elements
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length
occupied
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percentage
of sequence
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|
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number of
elements
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length
occupied
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percentage
of sequence
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SINEs:
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8
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367 bp
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0.00%
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SINEs:
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0
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0 bp
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0.00%
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ALUs
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0
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0 bp
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0.00%
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ALUs
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0
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0 bp
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0.00%
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MIRs
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3
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178 bp
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0.00%
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MIRs
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0
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0 bp
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0.00%
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LINEs:
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106
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24,847 bp
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0.05%
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LINEs:
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14
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1,154 bp
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0.01%
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LINE1
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9
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584 bp
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0.00%
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LINE1
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4
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245 bp
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0.00%
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LINE2
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4
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223 bp
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0.00%
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LINE2
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3
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146 bp
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0.00%
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L3/CR1
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14
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1,645 bp
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0.00%
|
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L3/CR1
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1
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79 bp
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0.00%
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LTR elements:
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24
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2,414 bp
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0.01%
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|
LTR elements:
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1
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38 bp
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0.00%
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ERVL
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1
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63 bp
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0.00%
|
|
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ERVL
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0
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0 bp
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0.00%
|
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ERVL-MaLRs
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0
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0 bp
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0.00%
|
|
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ERVL-MaLRs
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0
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0 bp
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0.00%
|
|
ERV_classI
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18
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1,872 bp
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0.00%
|
|
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ERV_classI
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1
|
38 bp
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0.00%
|
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ERV_classII
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0
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0 bp
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0.00%
|
|
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ERV_classII
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0
|
0 bp
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0.00%
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DNA elements:
|
177
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20,735 bp
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0.05%
|
|
DNA elements:
|
18
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2,687 bp
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0.03%
|
|
hAT-Charlie
|
60
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7,524 bp
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0.02%
|
|
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hAT-Charlie
|
10
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2,150 bp
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0.03%
|
|
TcMar-Tigger
|
42
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5,299 bp
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0.01%
|
|
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TcMar-Tigger
|
2
|
71 bp
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0.00%
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Unclassified:
|
2
|
94 bp
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0.00%
|
|
Unclassified:
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0
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0 bp
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0.00%
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Total interspersed repeats:
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48,457 bp
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0.11%
|
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Total interspersed repeats:
|
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3,879 bp
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0.05%
|
Small RNA:
|
99
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11,300 bp
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0.02%
|
|
Small RNA:
|
2
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200 bp
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0.00%
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Satellites:
|
2
|
326 bp
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0.00%
|
|
Satellites:
|
0
|
0 bp
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0.00%
|
Simple repeats:
|
0
|
0 bp
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0.00%
|
|
Simple repeats:
|
0
|
0 bp
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0.00%
|
Low complexity:
|
0
|
0 bp
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0.00%
|
|
Low complexity:
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0
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0 bp
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0.00%
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D. Mito-genome Map

(4) Microsatellite È帱º
A. SSR statistics
Motif(-mer)
|
2
|
3
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4
|
5
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6
|
7
|
8
|
9
|
10
|
Total
|
Á¦ÁÖ¹è²Å´ÞÆØÀÌ
|
5,633
|
2,311
|
626
|
17
|
14
|
5
|
7
|
1
|
3
|
8,617
|
B. ¹ÙÄڵ弿
>Á¦ÁÖ¹è²Å´ÞÆØÀÌ(Aegista chejuensis)
ACTTTATATATATTATTTGGTGTTTGATGTGGAATAGTTGGATCTGGTTTATCATTATTA
ATCCGTATAGAATTAGGAACTTGTAATGTATTAAGCGATAGTCATTTTTTTAATGTTATT
GTTACAGCACATGCATTTGTAATAATCTTCTTTATAGTAATACCAATCATGATTGGTGGT
TTTGGAAACTGAATAGTTCCATTACTAATTGGGGCTCCTGATATAAGTTTTCCACGAATA
AATAATATAAGATTTTGATTATTACCACCTTCATTTATCTTATTAATTTCAAGAAGATTA
GTTGAAGGTGGGGCTGGAACTGGGTGAACTGTATATCCACCTTTAAGTTCAATTATAGGT
CATAGAGGTGCTTCAGTAGATTTAGCAATTTTTTCTTTACATTTAGCTGGAATATCATCT
ATTTTAGGTGCAATTAATTTTATTACTACAATTTTTAATATACGTAGGCCAGGAAACTCT
ATAGAACGATTAAGATTATTTGTATGATCTATTTTAATTACAGTATTTTTATTACTTCTA
TCATTACCTGTTTTAGCAGGTGCTATTACAATATTATTAACTGATCGAAATTTTAATACT
TCATTTTTTGATCCTGCAGGAGGAGGAGATCCTATTTTATATCAGCATTT
Lozekia deubeli : family ºÎÅÍ ´Ù¸§
Baudinella thielei :¡¡superfamily ºÎÅÍ ´Ù¸§

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